STAGE Master 2

Date de mise à jour de l’offre

Université Paris Descartes :

Université Paris Descartes Etablissement public à caractère scientifique Culturel et Professionnel

Description de la mission

GABAergic interneurons play a critical role in neural network activity and behavior. During cortical development, these neurons are born from the medial ganglionic eminence (MGE) that produces 60% of cortical interneurons in mice. The caudal ganglionic eminence (CGE) is the second source producing 30% of interneurons, and the embryonic preoptic area (POA) is a third source contributing with 10%. Interestingly, as for 70% of the interneurons, the first wave of oligodendrocyte precursor cells (firstOPCs) is generated from the MGE and POA. Therefore, all firstOPCs and 70% of cortical interneurons are born from the same embryonic origins. The convergence in the development of firstOPCs and interneurons suggest possible interactions between these two cell types that might participate to cortical circuit construction and maturation. A major characteristic of OPCs is that they are the only glial cell type receiving bona fide functional synapses from neurons in the CNS. We previously showed that GABAergic interneurons constitute the major synaptic input of OPCs in the mouse somatosensory cortex. This neuron-glia synaptic innervation is highly regulated in time and space and reaches a peak of connectivity at postnatal day 10. Interestingly, firstOPCs are supposed to disappear by programmed cell death at this developmental stage and thus, it is considered that this specific OPC population does not play a role at postnatal stages. However, our unpublished data show that not all firstOPCs dye and that the surviving population preferentially interacts with GABAergic interneurons sharing a common embryonic origin. These results reveal an exquisite postnatal relationship between interneurons and firstOPCs favored by the same origin and uncover an unprecedented lineage-related cell interaction during a critical postnatal period for circuit formation and myelination in the cortex. In this project, we will evaluate whether the interactions between firstOPCs and their lineage-related interneurons contribute to the correct assembly and myelination of cortical neuronal networks. To test this, we have already generated mouse models of OPC survival. The aim of this M2 training will be to analyze the impact of firstOPC survival on cortical circuit development and myelination using electrophysiology (patch-clamp) and immunostainings on these models. This study has potential implications in pathological conditions such as psychiatric neurodevelopmental disorders.

Profil recherché

A student having a Master 1 in Neurobiology and motivated to do Neurophysiology.

Niveau de qualification requis

Bac + 4/5 et +
  • Employeur
    Université Paris Descartes
  • Secteur d’activité de la structure
    Enseignement - Formation - Recherche
  • Effectif de la structure
    Plus de 250 salariés
  • Site internet de la structure
  • Type de stage ou contrat
    Stage d'immersion en milieu professionnel dans le cadre de la formation professionnelle continue
  • Date prévisionnelle de démarrage
  • Durée du stage ou contrat
    Plus de 4 mois et jusqu'à 6 mois
  • Le stage est-il rémunéré ?
  • Niveau de qualification requis

    Bac + 4/5 et +
  • Lieu du stage
    Institut de Psychiatrie et Neurosciences de Paris
    102, rue de la Santé
  • Accès et transports
    Transport Parisien