STAGE M2 Internship Proposal 2022-2023: Diamond Quantum Microscopy of Neuronal Electrical Activity

Date de mise à jour de l’offre

Centre national de la recherche scientifique (CNRS) IDF Villejuif :

Le Centre national de la recherche scientifique est un organisme public de recherche pluridisciplinaire placé sous la tutelle du ministère de l’Enseignement supérieur, de la Recherche et de l’Innovation. Missions : faire de la recherche scientifique, valoriser les résultats, partager les connaissances, former par la recherche, contribuer à la politique scientifique.

Description de la mission

How electrical signals propagate in a neuron and across neuronal assemblies is still poorly understood. Theoretical modeling struggles to explain the subtle nature of neuron network connectivity and experimental measurements of individual neuron electrical signals across large networks remain elusive. Whilst many techniques exist to measure neuron activity, none of them can perform at high spatiotemporal resolution with wide-field imaging capacity and low invasiveness. To achieve this, we are developing a quantum sensor based on the optically detectable electron spin magnetic resonance (ODMR) of negatively charged nitrogen-vacancy (NV-) center in diamond nanostructures (Fig. 1). This three-year duration project (2022-2025) is supported by a Human Frontier Science Program (HFSP) cross-disciplinary fellowship.
The first steps of this project consists in 1) fabricating the diamond nanopillar array with NV- centers at the pillar’s tips (collab. with J.-F. ROCH [LuMIn] and Xavier CHECOURY [C2N, Palaiseau]) on which neuron grow efficiently as already established (Fig. 1a); and 2) constructing a “quantum microscope combining wide-field electrometry (continuous wave or pulsed ODMR) and live primary neuron culture imaging at large magnification. This experimental effort will be matched with theoretical modeling of the neuron electrodynamics in the presence of a nanopillar.

Profil recherché

The master internship will focus on the construction of novel wide-field “quantum microscope”. The candidates should have a good background in quantum physics with an interest in both experimental and theoretical approaches. Understanding of neuroscience concepts, nanofabrication steps, and Python programming is also very helpful but not mandatory. Finally, candidates should have a good grasp of English.

Niveau de qualification requis

Bac + 4/5 et +
  • Employeur
    Centre national de la recherche scientifique (CNRS) IDF Villejuif
  • Secteur d’activité de la structure
    Enseignement - Formation - Recherche
  • Effectif de la structure
    Plus de 250 salariés
  • Type de stage ou contrat
    Stage pour lycéens et étudiants en formation initiale
  • Date prévisionnelle de démarrage
  • Durée du stage ou contrat
    Plus de 4 mois et jusqu'à 6 mois
  • Le stage est-il rémunéré ?
    Oui
  • Niveau de qualification requis

    Bac + 4/5 et +
  • Lieu du stage
    Laboratoire LuMIn, ENS Paris-Saclay
    4 avenue des Sciences
    91190 GIF SUR YVETTE
  • Accès et transports
    RATP, Bus (https://www.ratp.fr/)