STAGE Innate sensing of HIV by cGAS and antiviral defenses in immune cells

Date de mise à jour de l’offre

Université Paris Descartes :

Université Paris Descartes Etablissement public à caractère scientifique

Description de la mission

The general goal of the project is to understand the principles that regulate immunity and antiviral defenses, using HIV as a model.
The lab is interested in understanding the general principles of innate and adaptive immunity, using HIV and dendritic cells. Innate immunity is an ancient and universal form of defense against pathogens such as viruses. In mammals, dendritic cells play a central role in controlling innate immune responses. Dendritic cells express a unique machinery to sense pathogens and orchestrate activation of the adaptive immune system. However, how dendritic cells sense viruses such as HIV and how this sensing can contribute to immune protection is not well understood. As a consequence, the ability to exploit innate immunity against HIV in therapeutics or prophylactic vaccines has yet to be achieved. Recently, we have demonstrated that dendritic cells can sense HIV infection thanks to the important cytosolic DNA sensor cGAS. Following on this work, the lab has now developed several new tools to analyze the innate and adaptive immunity against HIV in the different target immune cells of the virus (dendritic cells, macrophages, T cells).
The project for the M2 internship will focus on novel molecular mechanisms of cGAS-mediated sensing and responses. In particular, the M2 student will investigate the signaling mechanism of cGAS in target immune cells in reponse to DNA and HIV and/or antiviral defenses induced by cGAS against HIV (including restriction and resistance factors).

Profil recherché

The laboratory employs multidisciplinary approaches including human immunology (cell sorting, high speed cytometry), virology (HIV production and infections), bioinformatics (gene expression analysis), screening, molecular biology (DNA constructions, mutagenesis), cellular biology (live & confocal microscopy) and access to samples form infected patients.
The candidate will benefit from strong interactions with other projects in the team and with other projects in the department. The Institut Curie provides state-of-the-art equipment and a strong multidisciplinary environment. The highly motivated candidate should have previous experience in immunology and/or virology.

Niveau de qualification requis

Bac + 4/5 et +
  • Employeur
    Université Paris Descartes
  • Secteur d’activité de la structure
    Enseignement - Formation - Recherche
  • Effectif de la structure
    Plus de 250 salariés
  • Type de stage ou contrat
    Stage pour lycéens et étudiants en formation initiale
  • Date prévisionnelle de démarrage
  • Durée du stage ou contrat
    Plus de 4 mois et jusqu'à 6 mois
  • Le stage est-il rémunéré ?
  • Niveau de qualification requis

    Bac + 4/5 et +
  • Lieu du stage
    12 rue Lhomond
  • Accès et transports